Restriction enzymes
Most restriction enzymes used in cloning recognize which kind of DNA sequence?
Answer A: explanation for every choice
A
Correct. Typical sites are 4–8 base pairs that read the same 5′→3′ on both strands. Staggered cuts leave complementary overhangs.
B
Recognition sites are short and specific.
C
Restriction enzymes cut double-stranded DNA.
D
Bacteria methylate their own restriction sites to protect them. These enzymes don't target eukaryotic promoters.
TakeawayRestriction sites: short palindromes. Sticky ends let fragments be joined by ligase.
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Reviewed 2026-11-05 by a medical student. Spot an error? Report it with question ID bb129.