Substrate-level vs. oxidative phosphorylation
Which enzyme produces ATP by substrate-level phosphorylation?
Answer B: explanation for every choice
A
ATP synthase makes ATP by oxidative phosphorylation, driven by the proton gradient.
B
Correct. Phosphoglycerate kinase transfers a phosphate directly from 1,3-bisphosphoglycerate to ADP in glycolysis.
C
Complex I transfers electrons from NADH and pumps protons. It makes no ATP.
D
Complex IV transfers electrons to O2, forming water, and pumps protons. It makes no ATP.
TakeawaySubstrate-level: a phosphate is handed directly from a high-energy substrate to ADP. Oxidative: ATP synthase, powered by the proton gradient.
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Reviewed 2026-11-05 by a medical student. Spot an error? Report it with question ID bb12.